This chapter explains the technology and chemistry behind the ADVIA Centaur^® XP high throughput, random-access analyzer for the quantitative determination of a broad range of analytes. The system includes an analyzer, disposable assay cuvettes, reagent packs and generic reagents. There is also a benchtop version (ADVIA Centaur CP). Samples may be loaded at any time and urgent requests can be run in Stat mode. The immunoassays are based on paramagnetic particle separation and acridinium ester-based chemiluminescence signal generation. Assay designs may be competitive or immunometric. The analyzer can run one- and two-step assay protocols. The features of the system are listed, and the design and principles explained. There are also sections on calibration, antibodies, separation, signal generation and detection, and data processing, including interfacing to laboratory information systems.

Jean Metzar is currently a Director of Product Management in the CAI Business Unit at Siemens Healthcare Diagnostics, Tarrytown, NY, USA. Jean has worked in clinical diagnostics arena since starting her career in the clinical laboratory in 1983. She has held roles in hospital lab management and in industry spanning both sales and marketing management.

ADVIA, Centaur^®, acridinium ester, microparticle, paramagnetic, chemiluminescence, random access, competitive, immunometric, calibration, antibodies, separation, signal generation, signal detection, Stat test, auto retest.